[GRG] Regenerative Med: 3D printing of blood vessels


(Regenerative Med: Better method of 3D printing for blood vessels.)

Hydrogel bioprinted microchannel networks for vascularization of
tissue engineering constructs

Luiz E. Bertassoni,abc Martina Cecconi,bc Vijayan Manoharan,bc
Mehdi Nikkhah,bc Jesper Hjortnaes,bc Ana Luiza Cristino,bc
Giada Barabaschi,bc Danilo Demarchi,d Mehmet R. Dokmeci,bc
Yunzhi Yange and Ali Khademhosseini*bcfgh


Vascularization remains a critical challenge in tissue engineering.
The development of vascular networks within densely populated and
metabolically functional tissues facilitate transport of nutrients
and removal of waste products, thus preserving cellular viability
over a long period of time. Despite tremendous progress in
fabricating complex tissue constructs in the past few years,
approaches for controlled vascularization within hydrogel based
engineered tissue constructs have remained limited. Here, we report
a three dimensional (3D) micromolding technique utilizing
bioprinted agarose template fibers to fabricate microchannel
networks with various architectural features within
photocrosslinkable hydrogel constructs. Using the proposed
approach, we were able to successfully embed functional and
perfusable microchannels inside methacrylated gelatin (GelMA), star
poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene
glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol)
diacrylate (PEGDA) hydrogels at different concentrations. In
particular, GelMA hydrogels were used as a model to demonstrate the
functionality of the fabricated vascular networks in improving mass
transport, cellular viability and differentiation within the cell-
laden tissue constructs. In addition, successful formation of
endothelial monolayers within the fabricated channels was
confirmed. Overall, our proposed strategy represents an effective
technique for vascularization of hydrogel constructs with useful
applications in tissue engineering and organs on a chip.

About Johnny Adams

My full-time commitment is to slow and ultimately reverse biological aging and age related decline for more years of healthy living. I’ve been active in this area since the 1970s, steadily building skills and accomplishments. I have a good basic understanding of the science of aging, and have many skills that complement those of scientists so they can focus on science to advance our shared mission. Broad experience Top skills: administration, management, information technology (data and programming), communications, writing, marketing, market research and analysis, public speaking, forging ethical win-win outcomes among stakeholders (i.e. high level "selling"). Knowledge in grant writing, fundraising, finance. Like most skilled professionals, I’m best described as a guy who defines an end point, then figures out how to get there. I enjoy the conception, design, execution and successful completion of a grand plan. Executive Director Gerontology Research Group (GRG). Manages Email discussion forum, web site, meetings and oversees supercentenarian (oldest humans, 110+ years) research. CEO / Executive Director Aging Intervention Foundation (dba for Carl I. Bourhenne Medical Research Foundation), an IRS approved 501(c)(3) nonprofit. http://www.AgingIntervention.org Early contributor to Supercentenarian Research Foundation. Co-Founder Geroscience Healthspan Forum. Active contributor to numerous initiatives to increase healthy years of life. Co-authored book on conventional, practical methods available today to slow the processes of aging – nutrition, exercise, behavior modification and motivation, stress reduction, proper supplementation, damage caused by improper programs, risk reduction and others. Fundamental understanding of, and experience in the genomics of longevity (internship analyzing and curating longevity gene papers). Biological and technical includes information technology, software development and computer programming, bioinformatics and protein informatics, online education, training programs, regulatory, clinical trials software, medical devices (CAT scanners and related), hospital electrical equipment testing program. Interpersonal skills – good communication, honest, well liked, works well in teams or alone. Real world experience collaborating in interdisciplinary teams in fast paced organizations. Uses technology to advance our shared mission. Education: MBA 1985 University of Southern California -- Deans List, Albert Quon Community Service Award (for volunteering with the American Longevity Association and helping an elderly lady every other week), George S. May Scholarship, CA State Fellowship. BA psychology, psychobiology emphasis 1983 California State University Fullerton Physiological courses as well as core courses (developmental, abnormal etc). UCLA Psychobiology 1978, one brief but fast moving and fulfilling quarter. Main interest was the electrochemical basis of consciousness. Also seminars at the NeuroPsychiatric Institute. Other: Ongoing conferences, meetings and continuing education. Aging, computer software and information technology. Some molecular biology, biotech, bio and protein informatics, computer aided drug design, clinical medical devices, electronics, HIPAA, fundraising through the Assoc. of Fundraising Professionals. Previous careers include: Marketing Increasing skill set and successes in virtually all phases, with valuable experience in locating people and companies with the greatest need and interest in a product or service, and sitting across the table with decision makers and working out agreements favorable to all. Information Technology: Management, data analysis and programming in commercial and clinical trials systems, and bioinformatics and protein informatics. As IT Director at Newport Beach, CA based technology organization Success Family of Continuing Education Companies, provided online software solutions for insurance and financial professionals in small to Fortune 500 size companies. We were successful with lean team organization (the slower moving competition was unable to create similar software systems). Medical devices: At Omnimedical in Paramount CA developed and managed quality assurance dept. and training depts. for engineers, physicians and technicians. Designed hospital equipment testing program for hospital services division. In my early 20’s I was a musician, and studied psychology and music. Interned with the intention of becoming a music therapist. These experiences helped develop valuable skills used today to advance our shared mission of creating aging solutions.
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