[GRG] Reg. Med.: stem cell therapy for OA

Stem cell + hyaluronic acid inhibit OA in mice


Cell therapy with G-CSF-mobilized stem cells in a rat
osteoarthritis model

Authors: Deng, Ming-Wei ; Wei, Shih-Jung; Yew, Tu-Lai ; Lee, Po-
Hui; Yang, Tzu-Yu ; Chu, Hen-Yi ; Hung, Shih-Chieh

G-CSF-mobilized peripheral blood stem cells (gm-PBSCs) offer a
convenient cell source for treatment of hematopoietic and vascular
disorders. Whether gm-PBSCs provide beneficial effects on skeleton
diseases, such as osteoarthritis (OA), remain unknown. This study
was undertaken to address the hypothesis that gm-PBSCs promote
articular regeneration in OA. Here, we studied the effect of
singledose intra-articular injection of gm-PBSCs from male donors
delivered in hyaluronic acid (HA) on papain-induced OA in the knee
joints of female Sprague Dawley (SD) rats. Contralateral OA knee
joints received single-dose HA alone serve as vehicle controls. We
evaluated the histologic changes in glycosaminoglycan, type II
collagen, type X collagen, modified Mankin score and cell apoptosis
rate in the articular cartilage of rat knees. We demonstrated that
gm-PBSCs were mobilized to the peripheral blood via G-CSF infusion
for 5 days in SDrats with increasing CD34+ percentage up to 55
folds. We showed that gm-PBSCs inhibit progression of papain-
induced OA via reducing articular surface irregularity,
fibrillation and erosion, preventing cellular necrosis and loss of
chondrogenic proteins, such as glycosaminoglycan and type II
collagen, at both 3 and 6 weeks after treatment. Moreover, gm-PBSCs
reduced modified Mankin scores and cellular apoptosis rate compared
with HA alone. Our findings demonstrate that HA plus gm-PBSCs,
rather than HA alone, inhibits progression of OA in rats in vivo.
Thus, intra-articular injection of gm-PBSCs is a convenient
protocol for treating OA with consistent beneficial effects.

DOI: http://ift.tt/1nnrrh4


About Johnny Adams

My full-time commitment is to slow and ultimately reverse age related functional decline to increase healthy years of life. I’ve been active in this area since the 1970s, steadily building skills and accomplishments. I have a good basic understanding of the science of aging, and have many skills that complement those of scientists so they can focus on science to advance our shared mission. Broad experience Top skills: administration, management, information technology (data and programming), communications, writing, marketing, market research and analysis, public speaking, forging ethical win-win outcomes among stakeholders (i.e. high level "selling"). Knowledge in grant writing, fundraising, finance. Like most skilled professionals, I’m best described as a guy who defines an end point, then figures out how to get there. I enjoy the conception, design, execution and successful completion of a grand plan. Executive Director Gerontology Research Group (GRG). Manages Email discussion forum, web site, meetings and oversees supercentenarian (oldest humans, 110+ years) research. CEO / Executive Director Carl I. Bourhenne Medical Research Foundation (Aging Intervention Foundation), an IRS approved 501(c)(3) nonprofit. http://www.AgingIntervention.org Early contributor to Supercentenarian Research Foundation. Co-Founder Geroscience Healthspan Forum. Active contributor to numerous initiatives to increase healthy years of life. Co-authored book on conventional, practical methods available today to slow the processes of aging – nutrition, exercise, behavior modification and motivation, stress reduction, proper supplementation, damage caused by improper programs, risk reduction and others. Fundamental understanding of, and experience in the genomics of longevity (internship analyzing and curating longevity gene papers). Biological and technical includes information technology, software development and computer programming, bioinformatics and protein informatics, online education, training programs, regulatory, clinical trials software, medical devices (CAT scanners and related), hospital electrical equipment testing program. Interpersonal skills – good communication, honest, well liked, works well in teams or alone. Real world experience collaborating in interdisciplinary teams in fast paced organizations. Uses technology to advance our shared mission. Education: MBA 1985 University of Southern California -- Deans List, Albert Quon Community Service Award (for volunteering with the American Longevity Association and helping an elderly lady every other week), George S. May Scholarship, CA State Fellowship. BA psychology, psychobiology emphasis 1983 California State University Fullerton Physiological courses as well as core courses (developmental, abnormal etc). UCLA Psychobiology 1978, one brief but fast moving and fulfilling quarter. Main interest was the electrochemical basis of consciousness. Also seminars at the NeuroPsychiatric Institute. Other: Ongoing conferences, meetings and continuing education. Aging, computer software and information technology. Some molecular biology, biotech, bio and protein informatics, computer aided drug design, clinical medical devices, electronics, HIPAA, fundraising through the Assoc. of Fundraising Professionals. Previous careers include: Marketing Increasing skill set and successes in virtually all phases, with valuable experience in locating people and companies with the greatest need and interest in a product or service, and sitting across the table with decision makers and working out agreements favorable to all. Information Technology: Management, data analysis and programming in commercial and clinical trials systems, and bioinformatics and protein informatics. As IT Director at Newport Beach, CA based technology organization Success Family of Continuing Education Companies, provided online software solutions for insurance and financial professionals in small to Fortune 500 size companies. We were successful with lean team organization (the slower moving competition was unable to create similar software systems). Medical devices: At Omnimedical in Paramount CA developed and managed quality assurance dept. and training depts. for engineers, physicians and technicians. Designed hospital equipment testing program for hospital services division. In my early 20’s I was a musician, and studied psychology and music. Interned with the intention of becoming a music therapist. These experiences helped develop valuable skills used today to advance our shared mission of creating aging solutions.
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