Re: [GRG] Major Mouse Testing Program — slides to be sent for the New Year!

On Jan 6, 2015, at 2:29 PM, Paul Wakfer wrote:

> On 15-01-06 11:19 AM, Craig Cooney wrote:
>> Dear Edouard,
>> Thank you for sharing your ppt with us. Because I didn’t see many specifics on how the studies will be run my recommendations include using purified diets like AIN-93 (not NIH-31) and to measure diet intake to determine treatment dosage and to monitor for inadverent CR effects. Also HET mice (Dave Harrison’s recommendation) would be best but another good option (less expensive) would be a well established cross (albeit if only an F1) such as B6C3F1 mice as Steve Spindler used in some of his studies. Inclusion of positive controls of CR mice in the diet studies and known life extending genes in the TG studies (like the Maria Blasco papers I sent today) is important.

> As Craig pointed out, CR controls are just as important as regular controls and all treatment groups should be monitored for crypto-CR since very often with anything at all foreign to a normal mouse food intake, there will be an automatic reduction of intake, which in itself will have a life/health extending effect if not too great.
> I would also suggest that all the mice be in similar enriched environments rather than the totally unrealistic small cages with nothing of interest to do.

Why not cages? Please explain your reasoning.

If your suggestion is correct, it will be more persuasive if explained. And people won’t be able to implement it correctly without understanding what they are doing and why.

(I say this not to criticize the lack of detail upfront. I have no objection there. You can’t and shouldn’t preemptively elaborate on everything. I am trying to explain why my question – and the additional details it asks for – are important to the anti-aging mission at GRG. Because these issues matter to how to do scientific experiments related to the GRG mission.)

One issue I see here is the concept that mice are like people with interests, rather than being like non-intelligent computer software with algorithms. Does anyone want to discuss that? Why do you think that? It is relevant to GRG mission b/c it’s relevant to how to do mice experiments which are important for anti-aging research.

Has a mouse ever done anything that contradicts the algorithmic not-people-like view of mice? I’m not aware of anything despite looking and asking various people in the past. And if all mouse behavior is compatible with the algorithmic view, why reject it?

This is all crucial to the GRG anti-aging mission because getting experiments right – in every little detail – can be the difference between correct and incorrect scientific conclusions. And also money is limited, so it’s important to know whether it’s necessary to spend money on something more than cages, and if so precisely what parameters it needs to have in detail (and note they need to be clearly defined and repeatable).

> In addition, the experiments should not be done with certain isolated ingredients that may increase requirements for other ingredients. Rather each diet needs to be a fully consistent whole to the extent that is known or even suspected. There are many such interactions, some of which are well known but many of which are not. Therefore, it would be a good idea to post to this list and elsewhere the precise entire set of parameters planned for each mouse group *before* any further action is taken, so that all such potentially negative problems can be eliminated (at least all that are currently known by members of the groups to which you post).

I agree about this, I think it makes sense. I also agree about the importance of controls, as explained above.

I would similarly urge that, before the experiment is done, write a very clear, exact and list of the mouse habitat parameters (if the interesting non-cage approach is taken). It needs to be be specified well enough to be reproducible by other research groups exactly enough to qualify as replicating the same experiment. And it needs to be carefully thought out enough to serve as a kind of standard that future research could use, so we don’t end up with a bunch of different mouse studies using different habitats making the results potentially not comparable. (Maybe there already is such a standard? I don’t know. If so it’d be great to post that for discussion of any flaws it might have that could be worth changing.)

Elliot Temple


About Johnny Adams

My full-time commitment is to slow and ultimately reverse age related functional decline to increase healthy years of life. I’ve been active in this area since the 1970s, steadily building skills and accomplishments. I have a good basic understanding of the science of aging, and have many skills that complement those of scientists so they can focus on science to advance our shared mission. Broad experience Top skills: administration, management, information technology (data and programming), communications, writing, marketing, market research and analysis, public speaking, forging ethical win-win outcomes among stakeholders (i.e. high level "selling"). Knowledge in grant writing, fundraising, finance. Like most skilled professionals, I’m best described as a guy who defines an end point, then figures out how to get there. I enjoy the conception, design, execution and successful completion of a grand plan. Executive Director Gerontology Research Group (GRG). Manages Email discussion forum, web site, meetings and oversees supercentenarian (oldest humans, 110+ years) research. CEO / Executive Director Carl I. Bourhenne Medical Research Foundation (Aging Intervention Foundation), an IRS approved 501(c)(3) nonprofit. Early contributor to Supercentenarian Research Foundation. Co-Founder Geroscience Healthspan Forum. Active contributor to numerous initiatives to increase healthy years of life. Co-authored book on conventional, practical methods available today to slow the processes of aging – nutrition, exercise, behavior modification and motivation, stress reduction, proper supplementation, damage caused by improper programs, risk reduction and others. Fundamental understanding of, and experience in the genomics of longevity (internship analyzing and curating longevity gene papers). Biological and technical includes information technology, software development and computer programming, bioinformatics and protein informatics, online education, training programs, regulatory, clinical trials software, medical devices (CAT scanners and related), hospital electrical equipment testing program. Interpersonal skills – good communication, honest, well liked, works well in teams or alone. Real world experience collaborating in interdisciplinary teams in fast paced organizations. Uses technology to advance our shared mission. Education: MBA 1985 University of Southern California -- Deans List, Albert Quon Community Service Award (for volunteering with the American Longevity Association and helping an elderly lady every other week), George S. May Scholarship, CA State Fellowship. BA psychology, psychobiology emphasis 1983 California State University Fullerton Physiological courses as well as core courses (developmental, abnormal etc). UCLA Psychobiology 1978, one brief but fast moving and fulfilling quarter. Main interest was the electrochemical basis of consciousness. Also seminars at the NeuroPsychiatric Institute. Other: Ongoing conferences, meetings and continuing education. Aging, computer software and information technology. Some molecular biology, biotech, bio and protein informatics, computer aided drug design, clinical medical devices, electronics, HIPAA, fundraising through the Assoc. of Fundraising Professionals. Previous careers include: Marketing Increasing skill set and successes in virtually all phases, with valuable experience in locating people and companies with the greatest need and interest in a product or service, and sitting across the table with decision makers and working out agreements favorable to all. Information Technology: Management, data analysis and programming in commercial and clinical trials systems, and bioinformatics and protein informatics. As IT Director at Newport Beach, CA based technology organization Success Family of Continuing Education Companies, provided online software solutions for insurance and financial professionals in small to Fortune 500 size companies. We were successful with lean team organization (the slower moving competition was unable to create similar software systems). Medical devices: At Omnimedical in Paramount CA developed and managed quality assurance dept. and training depts. for engineers, physicians and technicians. Designed hospital equipment testing program for hospital services division. In my early 20’s I was a musician, and studied psychology and music. Interned with the intention of becoming a music therapist. These experiences helped develop valuable skills used today to advance our shared mission of creating aging solutions.
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