Thank you for your interesting post!
It seems you are planning to gather an awful lot of data. Of course that has 2 sides to it; the downside is that each test increases the cost. The upside is that each “positive” test result adds weight to your hypothesis.
The only extra tests I thought you might consider might be for MCP-1 (aka CCL1), RANTES (aka CCL5) and CD294+ Th2 cells.
These have been shown to be correlated with increasing age in a few papers, but Mansfield et al (2012) Clin. & Exp. Immunol. 170:186-193 is relatively recent.
One more comment: I was puzzled by “different components will be given at different times before and after plasma exchange”…are you able to clarify what you meant here?
I wish you good luck with putting it all together. It soulds like ground-breaking work!
On Sat, Mar 21, 2015 at 5:23 AM, Dr. Harold Katcher wrote:
Dear GRG members,
Perhaps you can do me a favor – particularly those of you who are physicians. I am writing a research proposal involving people and I don’t know what the costs would be. Also, do you think that the evidence I collect would be strong enough to be evidence of rejuvenation. I’ve taken the most stringent criteria from a paper that included Tom Kirkwood – and from others. The initial exam is given, different components will be given at different times before and after plasma exchange. The best I could get on plasma exchange itself is about 1-5 thousand dollars per treatment. If anyone has further information on any aspect of what is presented below, I’d like to hear it. If anyone has anything to add that would firm up the results – well maybe we could add you onto the paper.
Below is my initial exam
Cytokines and antibodies (some may not be feasible the first 8 are required, 11 is highly preferred as are 11 – 15.
7. Soluble TNF-receptor (type I)
8. Soluble TNF-receptor (type II)
9. Autoantibodies/ – against a variety of factors (RF, ANA, anti-thyroid Ab)
10. Immune complexes
11. BNP (brain natriuretic protein)
12. granulocyte colony-stimulating factor (G-CSF)
13. CCL-11 (‘eotaxin’)
14. MIG (monokine induced by gamma interferon)
15. macrophage colony-stimulating factor (M-CSF)
16. Exosomes RNA content (if only – would require ‘deep sequencing’)
1. FACS for distribution of b-cells, t-cells (CD-3, CD-28) CD4+, CD25+, CD8+ CD28+
2. P16INK4a expression in T – cells
1. Insulin and glucose
3. Lipids and apolipoproteins (apo A1, apoB, apoC, apoE)
4. Testosterone (free)
5. Hemoglobin concentration
7. Free T3
8. Sedimentation rate
Other factors (cellular factors also in the blood (or liver, skin – we need an earlier marker than HSC rejuvenation)
2. Telomere length
4. miRNA expression profiling (qPCR)
3. BUN and creatinine
1. Cognitive ability/memory testing (computerized?)
2. Physical strength, Up and Go
5. Age-specific quality of life scale
Senescent Symptoms to be photographed (with a ruler alongside)
1. Balding patterns
2. Age spots
3. Senile fold (earlobe)
Thanks for looking at it.