Re: [GRG] Combined Therapies mTERT + Plasma

Dear Harold,
Thank you for your interesting post!
It seems you are planning to gather an awful lot of data. Of course that has 2 sides to it; the downside is that each test increases the cost. The upside is that each “positive” test result adds weight to your hypothesis.
The only extra tests I thought you might consider might be for MCP-1 (aka CCL1), RANTES (aka CCL5) and CD294+ Th2 cells.
These have been shown to be correlated with increasing age in a few papers, but Mansfield et al (2012) Clin. & Exp. Immunol. 170:186-193 is relatively recent.
One more comment: I was puzzled by “different components will be given at different times before and after plasma exchange”…are you able to clarify what you meant here?
I wish you good luck with putting it all together. It soulds like ground-breaking work!
Cheers,
Thomas

On Sat, Mar 21, 2015 at 5:23 AM, Dr. Harold Katcher wrote:

Dear GRG members,
Perhaps you can do me a favor – particularly those of you who are physicians. I am writing a research proposal involving people and I don’t know what the costs would be. Also, do you think that the evidence I collect would be strong enough to be evidence of rejuvenation. I’ve taken the most stringent criteria from a paper that included Tom Kirkwood – and from others. The initial exam is given, different components will be given at different times before and after plasma exchange. The best I could get on plasma exchange itself is about 1-5 thousand dollars per treatment. If anyone has further information on any aspect of what is presented below, I’d like to hear it. If anyone has anything to add that would firm up the results – well maybe we could add  you onto the paper.

Below is my initial exam

Blood Tests: 

Cytokines and antibodies (some may not be feasible the first 8 are required, 11 is highly preferred as are 11 – 15.

1. CRP

2. TNF-alpha

3. TGF-beta

4. IL-10

5. IL-6

6. IL-1

7. Soluble TNF-receptor (type I)

8. Soluble TNF-receptor (type II)

9. Autoantibodies/ – against a variety of factors (RF, ANA, anti-thyroid Ab)

10. Immune complexes

11. BNP (brain natriuretic protein)

12. granulocyte colony-stimulating factor (G-CSF)

13. CCL-11 (‘eotaxin’)

14. MIG (monokine induced by gamma interferon)

15. macrophage colony-stimulating factor (M-CSF)

16. Exosomes RNA content (if only – would require ‘deep sequencing’)

Lymphocytes

1. FACS for distribution of b-cells, t-cells (CD-3, CD-28) CD4+, CD25+, CD8+ CD28+

2. P16INK4a expression in T – cells

Metabolism

1. Insulin and glucose

2. IGF-1

3. Lipids and apolipoproteins (apo A1, apoB, apoC, apoE)

4. Testosterone (free)

5. Hemoglobin concentration

6. DHEA

7. Free T3

8. Sedimentation rate

Other factors (cellular factors also in the blood (or liver, skin – we need an earlier marker than HSC rejuvenation)

1. Telomerase

2. Telomere length 

3. MMP-9

4. miRNA expression profiling (qPCR)

Safety labs

1. CBC

2. LFT’s

3. BUN and creatinine

Functional Testing

1. Cognitive ability/memory testing (computerized?)

2. Physical strength, Up and Go

3. Presbyopia 

4. FEV

5. Age-specific quality of life scale

Senescent Symptoms to be photographed (with a ruler alongside)

1. Balding patterns

2. Age spots

3. Senile fold (earlobe)

Thanks for looking at it.

Sincerely,

Harold Katcher

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About Johnny Adams

My full-time commitment is to slow and ultimately reverse age related functional decline to increase healthy years of life. I’ve been active in this area since the 1970s, steadily building skills and accomplishments. I have a good basic understanding of the science of aging, and have many skills that complement those of scientists so they can focus on science to advance our shared mission. Broad experience Top skills: administration, management, information technology (data and programming), communications, writing, marketing, market research and analysis, public speaking, forging ethical win-win outcomes among stakeholders (i.e. high level "selling"). Knowledge in grant writing, fundraising, finance. Like most skilled professionals, I’m best described as a guy who defines an end point, then figures out how to get there. I enjoy the conception, design, execution and successful completion of a grand plan. Executive Director Gerontology Research Group (GRG). Manages Email discussion forum, web site, meetings and oversees supercentenarian (oldest humans, 110+ years) research. CEO / Executive Director Carl I. Bourhenne Medical Research Foundation (Aging Intervention Foundation), an IRS approved 501(c)(3) nonprofit. http://www.AgingIntervention.org Early contributor to Supercentenarian Research Foundation. Co-Founder Geroscience Healthspan Forum. Active contributor to numerous initiatives to increase healthy years of life. Co-authored book on conventional, practical methods available today to slow the processes of aging – nutrition, exercise, behavior modification and motivation, stress reduction, proper supplementation, damage caused by improper programs, risk reduction and others. Fundamental understanding of, and experience in the genomics of longevity (internship analyzing and curating longevity gene papers). Biological and technical includes information technology, software development and computer programming, bioinformatics and protein informatics, online education, training programs, regulatory, clinical trials software, medical devices (CAT scanners and related), hospital electrical equipment testing program. Interpersonal skills – good communication, honest, well liked, works well in teams or alone. Real world experience collaborating in interdisciplinary teams in fast paced organizations. Uses technology to advance our shared mission. Education: MBA 1985 University of Southern California -- Deans List, Albert Quon Community Service Award (for volunteering with the American Longevity Association and helping an elderly lady every other week), George S. May Scholarship, CA State Fellowship. BA psychology, psychobiology emphasis 1983 California State University Fullerton Physiological courses as well as core courses (developmental, abnormal etc). UCLA Psychobiology 1978, one brief but fast moving and fulfilling quarter. Main interest was the electrochemical basis of consciousness. Also seminars at the NeuroPsychiatric Institute. Other: Ongoing conferences, meetings and continuing education. Aging, computer software and information technology. Some molecular biology, biotech, bio and protein informatics, computer aided drug design, clinical medical devices, electronics, HIPAA, fundraising through the Assoc. of Fundraising Professionals. Previous careers include: Marketing Increasing skill set and successes in virtually all phases, with valuable experience in locating people and companies with the greatest need and interest in a product or service, and sitting across the table with decision makers and working out agreements favorable to all. Information Technology: Management, data analysis and programming in commercial and clinical trials systems, and bioinformatics and protein informatics. As IT Director at Newport Beach, CA based technology organization Success Family of Continuing Education Companies, provided online software solutions for insurance and financial professionals in small to Fortune 500 size companies. We were successful with lean team organization (the slower moving competition was unable to create similar software systems). Medical devices: At Omnimedical in Paramount CA developed and managed quality assurance dept. and training depts. for engineers, physicians and technicians. Designed hospital equipment testing program for hospital services division. In my early 20’s I was a musician, and studied psychology and music. Interned with the intention of becoming a music therapist. These experiences helped develop valuable skills used today to advance our shared mission of creating aging solutions.
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