Benefits of Exercise and Home Gym

There are a great many benefits of exercise – particularly weight/resistance training and high intensity interval training.

You can benefit from my experience in buying home exercise equipment below.

Recently I bought some home gym equipment at bargain rates.   The intention was to save about 50-70 hours of drive time per year to and from the gym – as well as reduce risk of getting the flu.

Then the coronavirus hit.   Surprise!  Practical home gym equipment seems to be in short supply these days.

GET PHYSICIANS APPROVAL FOR PHYSICAL EXERCISE.
And learn proper training techniques.

Here’s what I use.  It lets me do almost everything done at a gym, plus some others you can’t.

1) Adjustable weight dumbbells

2) Life Fitness ParaBody GS2 Multi-gym with Leg Press
Picked this up for $350 bucks on Craigslist!
Delivery and setup are a pretty big deal.  The guy had a truck so he delivered it and set it up at no charge.
https://shop.lifefitness.com/g2-home-gym-with-leg-press

3) Bench with incline and decline
Bought this for about $100 bucks on Craigslist!

Not this one, but kind of like it
https://shop.lifefitness.com/hammer-strength-multi-adjustable-bench

4) TRX style resistance straps
They can be kind of expensive but you can get them cheap like this one
https://www.wish.com/product/5c247559ae599f45bde6af20?share=web

5) Elliptical machine – Pro-Form Hybrid Trainer.  No optional screen.  These get fancy with all kinds of programmed drills. I wanted it for high intensity interval training, and consistent resistance and measurement of the high physiological component of exercise stamina as part of the biomarker/objective measurement system.

Picked it up for $150 bucks on Craigslist!  It has both elliptical and stationary bike.
Transporting it can be done by two people, and it may be necessary to rent a truck or van. The guy had a truck so he delivered it for another $50.
https://www.proform.com/hybrid-trainers/hybridtrainer

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Longevity 2020 Online Conference Presentation

Here’s a presentation I made on May 1, 2020 at the Longevity 2020 Online Conference Presentation.

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Microbiome pilot study with Lactobacillus Reuteri 6475

This pilot study originally done and data collected in 2015.  A new method to analyze results became available in 2019.

It’s actually a microbiome experiment to find out whether some earlier work in animals and humans would translate to humans (the two of us in this pilot/small study).

It’s another example of the importance of the microbiome in health and longevity.

Let’s talk if you plan to recreate this, or do something like it.  There can be positive and negative effects, and different people may react in different ways.

BACKGROUND

Studies had shown that the gut microbe Lactobacillus Reuteri 6475 upregulated oxytocin (OT) production, and probably had some other unknown activity, resulting in positive effects.

Biologicals of this type were difficult to get, but since we have a 501(c)(3) nonprofit foundation I was able to complete a rigid qualification process to purchase.

For our small/pilot study, Bill cultured the 6475 and we both ate a cup 2x daily.  It tasted OK.

In 2015 our biomarker and objective measure testing was not nearly as developed as now.

We had some lab biomarkers before and after, including complete blood count/metabolic panel (CBC) and C-Reactive Protein (CRP), and grip strength.

Tests to directly measure OT would have been particularly useful.  We later learned about a new test for OT from Meridian Labs.

We achieved at least some positive objectively measured results (one was outstanding), and some positive subjective effects that some might say indicated better clearance of brain waste components while sleeping, and some negative.

Some positive effects of oxytocin and L reuteri reported in literature, mostly in animals but some humans:

  • Improved aged muscle stem cell functionOxytocin (OT) injected subcutaneously improved aged muscle stem cell function
  • Oxytocin – Attenuates atherosclerosis and inflammation
  • Oxytocin – Wound healing in Humans
  • L reuteri – Reduced intestinal inflammation
  • Lactobacillus reuteri 6475 Oxytocin – Wound healing is twice as fast
  • Lactobacillus reuteri 6475 – Decreases gut inflammation Reverses or prevents bone loss, osteoporosis
  • Lactobacillus reuteri 6475 – Maintains young testosterone levels & testicular size

After we consulted with age management physicians and could not uncover any objective changes in the lab work.  Some changes were noted that were probably random and certainly not statistically significant.  One with training in neurology and neuroendocrine said although no harm was done, it might be similar to a drug.

But years later and through the outstanding work of Morgan Levine to correlate standard CBC/metabolic panel and CRP data with computed DNA methylation age, AND the work of our member John Cramer to create a spreadsheet so we can use Levine’s method –

I plugged the CBC and CRP data into the “LevineCramer” spreadsheet to calculate phenotypic age.

After the treatment my phenotypic age had decreased from 63.25 to 58.65 – 4.6 years.

And subjectively something was definitely going on.

At the beginning we both had robust, active dreams through much of the night.  There was a kind of similar negative recurring theme to mine.  Appeared oxytocin or something else was getting into the brain.

For a while more thirsty – especially at night.  Less later.

Pretty sure more athletic stamina when walking (even after working hard all day)

Maybe more energy – maybe not.

Definitely higher libido

Felt revved up — Songs going through my head

When meditating seemed less able to get into a meditative state

But I was working very hard, and living in an old motorhome and was kind of roughing it.

After discontinuing I became kind of sad for a couple days.  I do NOT ever get sad or depressed in that way.

Bill’s notes are no longer available.

There’s a company called BioGaia that offered a product called “Gastrus”  It contained L reuteri 6475 and 17938.

https://www.iherb.com/pr/BioGaia-Gastrus-For-GI-Tract-Mandarin-Flavored-30-Chewable-Tablets/80974

It wasn’t sold in the US at the time but I used some resourcefulness to get it out of South Korea where it was being tested.

The “formal” part of the small study had ended and I switched from the yogurt to the Biogaia Gastrus.  Living conditions were changing and here it gets a little murky.

—–

WHETHER TO CONTINUE OR NOT

After the end of the 1 month small study I needed to return from the San Francisco Bay Area to Orange County.  The Gastrus didn’t seem to have any effects so decided other action items were more important that culturing it on my own so based on the resulting information from limited objective measures at the time I decided not to continue.

—–

RECENT TEST WE DID WITH 3 GRG MEMBERS

Maybe they would like to comment.

Recently in addition to “Gastrus” (contained 6475 and 17938) BioGaia started selling Osfortis with only 6475.

https://www.biogaia.com/product/biogaia-osfortis/

Three members took the recommended dose 1 tablet 2x daily of Osfortis for a month, and had CBC and CRP tests before and just before ending.

They did report any negative side effects so carried it out to the full 1 month.

They did not show a significant positive effects or reduction in phenotypic age.  One’s LevineCramer markers went in the right direction, and others went in the undesirable direction, with result that was actually about 2-1/2 yr older.

Johnny

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Attn Scientists who are Aging SOLUTION CREATORS

Attn Scientists who are Aging SOLUTION CREATORS

If you are developing a new aging intervention therapy with exceptional promise to help us live longer and healthier lives, then my wide network may help in many ways including:

  • funding as well as patent, business, legal, scientific, promotion among others.

Conversations are CONFIDENTIAL.
You stay in control.
We’re in this to solve aging.

Call me at (949) 922-9786
or email JAdams -at- AgingInterventionFoundation . org

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Online Longevity Conference Worldwide: 27 April – 01 May 2020

Online Longevity Conference

27 April – 01 May 2020

An outstanding organization, Longevity Technology – headed by Phil Newman and a highly talented team – is organizing an innovative 5 day ALL ONLINE conference with outstanding content.

Like many of us, they’re disappointed that events have been canceled, funding rounds postponed, and new knowledge is going unshared.

So they thought: everyone’s at home, even world experts, so let’s get together and keep the Longevity sector on track.

They plan to have a freemium model: everyone will get to see content for FREE; some features like interactive Q&A, networking and cocktails will be paywalled.

Web site:
https://www.longevity2020.com/

Check out Longevity Technology and consider subscribing.
https://www.longevity.technology/

Johnny

John M. “Johnny” Adams
Executive Director Gerontology Research Group
JAdams -at- grg.org
(949) 922-9786 cell
~~~~
CEO / Exec. Director  Aging Intervention Foundation / Bourhenne Medical Research Foundation
JAdams -at- AgingInterventionFoundation.org
https://www.aginginterventionfoundation.org/AgingInterventionProgram.pdf
http://www.AgingIntervention.org
http://www.AgingIntervention.org/JohnnyAdamsBackgroundSummary.htm

 

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Senolytics — opportunity and caution

Senolytics
Johnny Adams
http://www.AgingIntervention.org
JAdams@AgingInterventionFoundation.org
(949) 922-9786
https://www.aginginterventionfoundation.org/Senolytics.pdf

Constantly improved. Check for updates with the link above. You may not have the current version.
Last updated March 8 2020 10:35 am.

_______________________

Friendly Disclaimer: These are ideas that I use in my own age management program. It’s not my intention to provide specific medical advice, but rather to provide others with information to better understand their health. This is not medical advice including diagnosis and treatment. Always seek the advice of a trained health professional for medical advice, diagnosis or treatment.

_______________________

Several GRG members have thoughtfully taken the time to send information about dasatinib, sources, and testing.

This is a serious matter. If misused, senolytics can be dangerous.

READ AND UNDERSTAND EVERYTHING BELOW – AND MUCH MORE – BEFORE YOU PROCEED WITH SENOLYTICS

If you’re not going to take the time to read and understand everything below –
and further educate yourself beyond my inadequate information provided for your benefit here

THEN YOU HAVE NO BUSINESS MESSING WITH SENOLYTICS.

You will benefit from our learning curve, which has been substantial.

Members quotes from submitted messages are attributed to them.

_______________________

Definition 

Senescence is the condition or process of deterioration with age. Senescent cells release a variety of factors such as pro-inflammatory cytokines and chemokines, which contribute to the physical dysfunction of tissues and organs during aging. They change cellular behavior for the worse, and destructively remodel the structure of tissues. Programmed cell death (apoptosis) often doesn’t turn on.

Senolytic therapy is an emerging aging intervention therapy. Senolytic drugs and over the counter compounds are intended to specifically target and eliminate senescent cells. It appears senescent cells are necessarily the ONLY targets of senolytics.

In animal studies eliminating senescent cells improved some physical dysfunctions and increased the healthy life span of mice, even the elderly mice. In mice, senolytic therapies have been shown to slow the progression of numerous age-related diseases.

Research in humans is underway, and results from early human trials have shown some positive effects.

Educate yourself as to the potential risks and benefits before undertaking it.

PLEASE SEND ME your information and results on human studies – informal, pilot or formal studies.

_______________________

Warnings

I suggest these warnings apply not only to dasatinib, which is a cancer drug – but to all senolytics including:
quercetin,
fisetin,
FOXO4-DRI,
theaflavins,
piperlongumine,
Tocotrienols – alone or with quercetin
L-Carnosine – sometimes taken with ALCAR and/or DMAE,
BCL-2 family inhibitors navitoclax / ABT263 and ABT-737,
Some other BCL-2 inhibitors like TW-37 are reported not to be senolytic, though, so it isn’t just any old BCL-2 inhibition that works,
BCL-XL inhibitors A1331852 and A1155463,
17-DMAG,
ABT-737,
ABT263,
navitoclax,
isoquercetin,
venetoclax,
sulforaphane,
BCL-2 protein family inhibitors in general,
HSP90 inhibitors

to name a few.

I believe this was true at one time, but may have changed:
Unity Biotechnology is deriving from navitoclax and using in their current studies.
Oisin Biotechnologies (DNA construct) lipid nanoparticle/p16-promoter/caspase DNA “drug”
SIWA Therapeutics (antibody).

* SENOLYTICS WARNING — Caution is advised re. excessive senolytics.
Note: What’s excessive may be different between individuals
Vince Giuliano advises senolytic signaling is critical for cell renewal – you need enough inflammation and senescence to signal for regeneration factors — so if you go overboard in senolytics therapy it’s bad, you will miss out on cell renewal.
http://www.anti-agingfirewalls.com/2018/09/02/aging-cell-and-tissue-repair-renewal-and-regeneration-inflammation-and-the-sasp

More is often not better. Quote by Reason: All senolytics, so far, look like things you would take once every few years at most. More won’t be any more effective than that one dose – it will kill the senescent cells it can kill the first time, and won’t be helpful again until more senescent cells turn up in volume.
http://www.fightaging.org/archives/2018/03/how-to-plan-and-carry-out-a-simple-self-experiment-a-single-person-trial-of-senolytic-peptide-foxo4-dri#caveats-in-more-detail

Senescent cell researcher Dorota Skowronska-Krawczyk PhD personally discourages us from having long treatments with senolytic drugs. In fact she suggests they should only be taken for short periods interspaced with longer recovery times.

Stan Goldfarb has decades of applied nutritional supplement and aging intervention experience. He advises: I think even 2.5mg per KG is a higher than I want to take, especially when combining it with EMIQ (which in itself has no bad side effects till very high doses). I weigh 137 and am going to take 100mg once only. You should also be taking at least 10000iu of D3 to complete apoptosis and don’t take any blood thinners such as aspirin or omega supplements as it has been proven to go after fat cells for several days before and after. Without doing all of this a person is simply taking an unnecessary risk. When I did my first test of this in 2015, there were some really sharp people to say exactly what to do and when. I don’t see that now with the current crop of people and it concerns me. People have died from overdosing this drug! I also remember that several people who did take multiple doses experienced minor problems after the second dose (especially flu like syptoms but not after the first. One group is saying take what you’re doing twice one week apart. This is potentially risky. The effect Dasatinib has lasts longer than many people seem to think and that is why I think a second dose just one week later makes no sense.

James Kirkland MD PhD recommends not evaluating senolytics on our own at this early stage.

Similar cautions apply to other aging intervention therapies. There’s a lot we don’t know about this new frontier.

Everyone is different – some may be harmed from senolytics, some may benefit from more senolytic, others may need very little (and will be harmed by more).

Individuals may respond differently to combinations.

Retreatment interval may vary from individual to individual.

From the Age Reversal Network (formerly Rescue Elders) document presented at RAADfest 2018:
Possible side effects: Mild flu symptoms, diarrhea, headache, fatigue for 12-24 hours.
Note from Johnny: And for some people who knows what else, and for how long.
Read the product information.

Take in the presence of a qualified medical doctor in case of severe allergic reaction.
Do not engage in strenuous exercise during, or for one week after, the treatment period.

That said . . .

Dasatinib and Quercetin act on different paths and are often taken together.

Dasatinib
They usually come in tablets. I have heard of 20, 50 and 70 mg.
I get 20 mg because it’s easier to measure the exact dose I want to take.
Do not cut, crush, or chew the tablets

Brands I have heard of:
Sprycel brand from Bristol-Myers Squib
Others from offshore sources.

Research grade can be good and comes highly recommended by one of our skilled MD associates. But you would need a scale and measuring small amounts can be tricky. I bought a scale and other items needed for this, but decided against it.
I DO NOT recommend research grade. But if you have a lot of experience, here you go https://lclabs.com/products/d-3307-dasatinib-free-base

READ THIS: Useful Sprycel dasatinib info from Bristol-Myers Squib
www.sprycel.com
It says:
• Do not cut, crush, or chew the tablets
• Do not drink grapefruit juice during treatment with SPRYCEL
• Do not take St. John’s wort during treatment with SPRYCEL

Grapefruit juice competes for the clearance channel with metformin, probably the same for dasatinib.
Hence it may be bad to take dasatinib with metformin. It appears some more experienced members of our community take grapefruit juice along with therapies – presumably for increased effectiveness and to save money. This sounds really tricky and I do not think I’ll try it.

_______________________

Dosing
I’m kind of sensitive to drugs, also conservative. When initially naïve once I took too much so called “anti aging” therapies and it made me seriously ill.

So first time around I took about 1/3 of the usual. A new and innovative lab test we developed showed positive results. But that’s just me.

Stan Goldfarb has decades of applied nutritional supplement and aging intervention experience. He advises: I think even 2.5mg per KG is a higher than I want to take, especially when combining it with EMIQ (which in itself has no bad side effects till very high doses) . I weigh 137 and am going to take 100mg once only . You should also be taking at least 10000iu of D3 to complete apoptosis and don’t take any blood thinners such as aspirin for several days before and after. Without doing all of this a person is simply taking an unnecessary risk. When I did my first test of this in 2015, there were some really sharp people to say exactly what to do and when. I don’t see that now with the current crop of people and it concerns me. People have died from overdosing this drug!

One group is saying take what you’re doing twice one week apart. This is high risk and probably even irrational.

Here’s dosing recommendations from the Age Reversal Network (formerly Rescue Elders) document presented at RAADfest 2018. I do not know how this was arrived at.  I am not endorsing it, it’s presented for informational purposes only:
One quercetin + dasatinib dose once a week for two weeks only (two total doses)

Quercetin
25 mg per kilogram of body weight, which is approx.:
100 pounds = 1125 mg

Dasatinib
2.5 mg per kilogram of body weight is approx.:
100 pounds = 112 mg

Hopefully you can do the math to arrive at your personal dosage.
Example, I weigh 146 lb, so dasatinib would be
112 mg for first 100 lb
Plus 46/100 x 112 = 51.52 (or .46 x 112)
= 163.52
REMEMBER Do not cut, crush, or chew the tablets
So round down (or up).

_______________________

Testing Pharmaceuticals Purchased Offshore and Elsewhere for Safety and Efficacy

I do not trust drugs from sources other than licensed pharmacies (and even licensed pharmacies have been known to get it wrong). I’ve been burned.

Counterfeit products is a huge international problem. It’s not just jeans, Gucci bags, Barbie dolls and GI Joes — others include counterfeit airplane parts, pharmaceuticals and other mission-critical products. Whatever makes money. A doctor once told me about fake Lasix medicine made seriously ill patients even sicker because it had been mixed in a container that had previously been used to mix pesticide, and had traces of the pesticide in the “Lasix”.

Appearance and packaging are VERY convincing. I read that a phoney Apple store was opened in China with real looking “Apple” products.

So I do not automatically trust that offshore suppliers will provide pharmaceuticals that are pure or contain the specified content.

Pete Cooperider replied to John Cramer’s post:
John, I see that the Bon Hoa pharmacy has several brands of dasatinib like Lucidas, Dasanix, Dasanat. Kindly tell us which one you used that you refer to as having been tested?

My response
Pete —
I suggest that testing one pill is pretty good evidence the rest in the bottle is OK, and the entire lot or batch is probably OK I guess – but does not necessarily mean that all tablets from that source are OK now, or will be into the future.

This concept also applies to nutritional supplements. Some suppliers use cheap ingredients that do not contain the specified amounts, and may even contain toxins.

According to some of my sources: So-called “Canadian” pharmacies are usually not in Canada – they’re usually in Asia, India or somewhere else. It is illegal for a Canadian pharmacy to ship to the US, with or without a prescription. Unless the web address has a “.pharmacy” extension it is not legitimate. The legitimate way to get a Canadian pharmaceutical is to go to Canada and get it from a Canadian doctor, then go to the pharmacy.

_______________________

We recently had dasatinib and rapamycin tested at Echelon Biosciences. I was very satisfied with the service and value.

They now have the qualitative test data and reports for dasatinib and rapamycin. Our foundation covered the initial cost of development, and you will be able to send your tablets to them for testing at a much lower cost.

For dasatinib, they have data for samples tested against a sample from a US pharmacy.
For rapamycin they have data for samples tested against a generic (known) sample AND a sample from a US pharmacy.

However, the topic of quantitative versus qualitative has arisen. Qualitative testing evaluates for purity, and that it contains the indicated pharmaceutical. Quantitative evaluates for the amount of the pharmaceutical.

Qualitative analysis (like Echelon did) is easier than quantitative, or active pharmaceutical ingredient (API) analysis.
Quantitative involves building a calibration curve. That may take 1 day, then the test may take 1 day
Total cost quoted from Emory Pharma for one instance: $5K. Others may vary.

Upon further investigation a knowledgeable scientist advised that qualitative comparative analysis (the kind Echelon did) – is all that’s really needed and will tell if there’s the same response in height and peak area – so will tell if pretty much the same amount.

All is beyond my pay grade so I have yet to completely understand it.

We are seeking further details and a conclusive answer as to whether qualitative or quantitative testing are required for our purposes.
Who will take on the task of getting a definitive answer?

Other testing labs can be found on Science Exchange http://www.scienceexchange.com .

Qualitative uses high-performance liquid chromatography (HPLC) and other methods. The generic (known) sample of dasatinib, was acquired through Sigma-Aldrich: CAS: 302962-49-8.
http://www.sigmaaldrich.com/catalog/buildingblock/product/matrixscientific/mat370173661?lang=en&region=US

Qualitative testing:
Echelon Biosciences now has testing data for dasatinib and rapamycin. They are available to test samples from other members of our community. My group has covered the initial cost, which is greater than the cost of testing additional samples.
 Contact me if you plan to utilize Echelon’s services. Inefficiency and confusion may be eliminated if you have me in the loop.
Johnny Adams JAdams@grg.org (949) 922-9786
Echelon Biosciences Inc.
Mark Nelson, PhD
675 Arapeen Drive Suite 302
Salt Lake City, UT 84108
mnelson@frontiersci.com
801-588-0455 ex 308

Quantitative testing
Emory Pharma

Home


Neeku (Niki) Mahdavian
Business Development Manager
Tel: (510) 899-8825
neeku@emerypharma.com

_______________________

Biomarkers and objective measures to evaluate senolytic therapy effectiveness in subjects/patients

Some useful ones are in part 3:

Click to access AgingInterventionProgram.pdf

Everyone is different – some may be harmed from senolytics, some may benefit from more senolytic, others may need very little (and will be harmed by more).

Retreatment interval may vary from individual to individual.

How do you know whether it’s working, and how much to take, and how often?

Current lab tests for this are entirely inadequate. Beta Galactosidase is not practical in humans.

Who has information on effective lab tests to evaluate dose, interval, etc.?
Please send it to JAdams@grg.org or call 949 922 9786.

We have found DNA methylation age from blood increases (goes in the wrong direction) after dasatinib plus quercetin. It’s uncertain whether this is an undesirable effect on one part of the system that accompanies the supposedly positive effects on others, or some kind of artifact, or what.

I worked with some top scientists at a major university research lab to develop an innovative biomarker test that was initially developed to measure cell senescence for before and after senolytic therapy.
It also applies to:
virtually any aging intervention therapy
and gene therapy.

We are now bringing it from the research lab to production mode. I will let you know when it’s ready for use in our community.

_______________________

Finally: now that you are a little more educated about the hazards and how complicated senolytic therapy is, on to sources.

But FIRST – to show you how much I care about you and really don’t want you to hurt yourself –

and I took a lot of time out of MY OWN aging intervention program to write all this. So you better read and understand it.

So another friendly reminder:

READ AND UNDERSTAND EVERYTHING ABOVE BEFORE YOU PROCEED WITH SENOLYTICS

If you’re not going to take the time to read and understand everything below – and further educate yourself beyond my inadequate information provided for your benefit here

THEN YOU HAVE NO BUSINESS MESSING WITH SENOLYTICS.

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Attn Research Scientists who are Aging SOLUTION CREATORS

Attention: Research Scientists who are Aging SOLUTION CREATORS

If you are developing a new aging intervention therapy with exceptional promise to help us live longer and healthier lives, then my wide network may help in many ways including funding, patent, business, legal, scientific, promotion among others.

Conversations are CONFIDENTIAL. You stay in control.  We’re in this to solve aging.

Call me at (949) 922-9786
or email JAdams -at- AgingInterventionFoundation.org

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